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The biocompatibility of Ti-OH-ePV. a) Schematic illustration of the co-culture model; b) Viability of <t>L929</t> cells after 24 h co-culture with Ti, Ti-OH, and Ti-OH-ePV samples; c) Live/dead fluorescence images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 200 μm); d) Cytoskeletal staining images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 100 μm); e) Hemolysis test results and f) quantitative hemolysis ratios of Triton X-100, PBS, Ti, Ti-OH, and Ti-OH-ePV groups; g) Immunohistochemical staining images of IL-4, IL-10, CD68, and IL-1β in rat subcutaneous tissues 7 days post-implantation (scale bar: 100 μm); h) Quantitative analysis of IL-4 and IL-10 expression; i) Quantitative analysis of CD68 and IL-1β expression; n = 3; ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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The biocompatibility of Ti-OH-ePV. a) Schematic illustration of the co-culture model; b) Viability of <t>L929</t> cells after 24 h co-culture with Ti, Ti-OH, and Ti-OH-ePV samples; c) Live/dead fluorescence images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 200 μm); d) Cytoskeletal staining images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 100 μm); e) Hemolysis test results and f) quantitative hemolysis ratios of Triton X-100, PBS, Ti, Ti-OH, and Ti-OH-ePV groups; g) Immunohistochemical staining images of IL-4, IL-10, CD68, and IL-1β in rat subcutaneous tissues 7 days post-implantation (scale bar: 100 μm); h) Quantitative analysis of IL-4 and IL-10 expression; i) Quantitative analysis of CD68 and IL-1β expression; n = 3; ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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The biocompatibility of Ti-OH-ePV. a) Schematic illustration of the co-culture model; b) Viability of L929 cells after 24 h co-culture with Ti, Ti-OH, and Ti-OH-ePV samples; c) Live/dead fluorescence images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 200 μm); d) Cytoskeletal staining images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 100 μm); e) Hemolysis test results and f) quantitative hemolysis ratios of Triton X-100, PBS, Ti, Ti-OH, and Ti-OH-ePV groups; g) Immunohistochemical staining images of IL-4, IL-10, CD68, and IL-1β in rat subcutaneous tissues 7 days post-implantation (scale bar: 100 μm); h) Quantitative analysis of IL-4 and IL-10 expression; i) Quantitative analysis of CD68 and IL-1β expression; n = 3; ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Bioactive Materials

Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

doi: 10.1016/j.bioactmat.2026.01.005

Figure Lengend Snippet: The biocompatibility of Ti-OH-ePV. a) Schematic illustration of the co-culture model; b) Viability of L929 cells after 24 h co-culture with Ti, Ti-OH, and Ti-OH-ePV samples; c) Live/dead fluorescence images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 200 μm); d) Cytoskeletal staining images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 100 μm); e) Hemolysis test results and f) quantitative hemolysis ratios of Triton X-100, PBS, Ti, Ti-OH, and Ti-OH-ePV groups; g) Immunohistochemical staining images of IL-4, IL-10, CD68, and IL-1β in rat subcutaneous tissues 7 days post-implantation (scale bar: 100 μm); h) Quantitative analysis of IL-4 and IL-10 expression; i) Quantitative analysis of CD68 and IL-1β expression; n = 3; ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Standard fibroblast cell line L929 fibroblasts and mouse macrophages (Raw264.7) were obtained from Jiangsu Keygen Biotech Co., Ltd. (China).

Techniques: Co-Culture Assay, Fluorescence, Staining, Immunohistochemical staining, Expressing

Evaluation of cell adhesion, proliferation, and pro-angiogenic potential of different samples. a) SEM images of Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 1 μm); b) Fluorescent images of L929 cells on Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d after co-culturing 72 h (scale bar: 200 μm); c) Fluorescent images of Matrigel tube formation by HUVECs treated with Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 200 μm); e) Scratch wound migration assay of HUVECs in the Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d groups (scale bar: 100 μm).

Journal: Bioactive Materials

Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

doi: 10.1016/j.bioactmat.2026.01.005

Figure Lengend Snippet: Evaluation of cell adhesion, proliferation, and pro-angiogenic potential of different samples. a) SEM images of Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 1 μm); b) Fluorescent images of L929 cells on Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d after co-culturing 72 h (scale bar: 200 μm); c) Fluorescent images of Matrigel tube formation by HUVECs treated with Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 200 μm); e) Scratch wound migration assay of HUVECs in the Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d groups (scale bar: 100 μm).

Article Snippet: Standard fibroblast cell line L929 fibroblasts and mouse macrophages (Raw264.7) were obtained from Jiangsu Keygen Biotech Co., Ltd. (China).

Techniques: Migration